Determination of histamine, methylhistamines and histamine—o-phthaldialdehyde complexes by two high-performance liquid chromatographic procedures : Application to biological samples |
| |
Authors: | J C Robert J Vatier B K Nguyen Phuoc S Bonfils |
| |
Institution: | Inserm U. 10, Unité de Recherche de Gastroentérologie, Hôpital Bichat, 170, Boulevard Ney, F-75877 Paris Cedex 18 France |
| |
Abstract: | Two high-performance liquid chromatographic procedures were proposed to measure histamine. The first, with UV detection and a strong acid cation exchanger (Partisil 10, SCX Whatman), made it possible to isolate histamine and some methylated derivatives. The second, with a C18 sorbent (μBondapak, Waters, 10 μm particle size) eluted with ion-pairing phases, made it possible to isolate the histamine—o-phthaldialdehyde complexes. This last procedure allied with a chromatographic purification step gave lower or identical amounts of histamine than those described in human urine (16 ± 7 μg per 24 h), canine whole blood (1.5 ± 1 ng/ml) and human gastric juice (2.3 ± 1.4 ng/ml). The two procedures gave the concentration of a histamine-like compound isolated from the antral mucosa. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|