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Bacillus daqingensis sp. nov., a halophilic,alkaliphilic bacterium isolated fromSaline-sodic soil in Daqing,China |
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| Authors: | Shuang Wang Lei Sun Dan Wei Baoku Zhou Junzheng Zhang Xuejia Gu Lei Zhang Ying Liu Yidan Li Wei Guo Shuang Jiang Yaqing Pan Yufeng Wang |
| Institution: | 1. Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin, 150086, P. R. China 2. Institute of Soil Fertilizer and Environmental Resources, Heilongjiang Academy of Agricultural Sciences/Key Lab of Soil Environment and Plant Nutrition of Heilongjiang Province/Heilongjiang Fertilizer Engineering Research Center/Scientific Observing and Experimental Station of Heilongjiang Farmland Conservation and Agricultural environment, Ministry of Agriculture, Harbin, 150086, P. R. China 3. School of Food Science and Engineering, Harbin Institute of Technology, Harbin, 150001, P. R. China 4. Institute of rural energy, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, P. R. China 5. Editorial Department of Journal of Northeast Agricultural University, Harbin, 150030, P. R. China 6. Pesticides Center, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, P. R. China |
| Abstract: | An alkaliphilic, moderately halophilic, bacterium, designated strain X10-1T, was isolated from saline-alkaline soil inDaqing, Heilongjiang Province, China. Strain X10-1T was determined to be a Gram-positive aerobe with rod-shaped cells. The isolate was catalase-positive, oxidase-negative, non-motile, and capable of growth at salinities of 0–16% (w/v) NaCl (optimum, 3%). The pHrange for growth was 7.5–11.0 (optimum, pH 10.0). The genomic DNA G+C content was 47.7 mol%. Itsmajor isoprenoid quinone was MK-7 and its cellular fatty acid profile mainly consisted of anteiso-C15:0, anteiso-C17:0, iso-C15:0, C16:0, and iso-C16:0. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences showed that X10-1T is a member of the genus Bacillus, being most closely related to B. saliphilus DSM15402T (97.8% similarity) and B. agaradhaerens DSM 8721T (96.2%). DNA-DNA relatedness to the type strains of these species was less than 40%. On the basis of the phylogenetic, physiological, and biochemical data, strain X10-1T represents a novel species of the genus Bacillus, for which the name Bacillus daqingensis sp. nov. is proposed. The type strain is X10-1T (=NBRC 109404T = CGMCC 1.12295T). |
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