Microclonal propagation and biological features of Scrophularia umbrosa dumort. Cultured in vitro

We have investigated the features of sterilization and germination of Scrophularia umbrosa seeds after different periods of storage as explants for in vitro culturing, as well as the details of morphogenesis and shoot regeneration, on the Murashige and Skoog medium supplemented with various plant hormones. We have examined the early stages of S. umbrosa development in vitro, and followed the introduction of microclones in the conditions closely resembling the natural habitat of the species. The full technological cycle of S. umbrosa micropropagation has been developed. We show that introduction of microclones obtained in vitro leads to the acceleration of the life cycle and the production of viable seeds.