Naturally occurring chlorophyll derivatives inhibit aflatoxin B1-DNA adduct formation in hepatoma cells |
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| Authors: | Ching-Yun Hsu Yue-Hwa Chen Pi-Yu Chao Chiao-Ming Chen Ling-Ling Hsieh Shene-Pin Hu |
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| Institution: | aCollege of Pharmacy, Taipei Medical University, Taipei 110, Taiwan;bChang-Gung Institute of Technology, Taoyuan 333,Taiwan;cSchool of Nutrition and Health Science, Taipei Medical University, Taipei 110, Taiwan;dDepartment of Food, Health and Nutrition Science, Chinese Culture University, Taipei 111, Taiwan;eDepartment of Public Health, Chang-Gung University, Taoyuan 333, Taiwan |
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| Abstract: | The inhibitory effects of four chlorophyll derivatives (chlorophyllide Chlide] a and b and pheophorbide Pho] a and b) on aflatoxin B1 (AFB1)-DNA adduct formation, and on the modulation of hepatic glutathione S-transferase (GST) were evaluated in murine hepatoma (Hepa-1) cells. Enzyme-linked immunosorbent assay showed that pretreatment with Chlide or Pho significantly reduced the formation of AFB1-DNA adducts, and that Pho was the most potent inhibitor. However, wash-out prior to adding AFB1 totally eliminated inhibition by Childe and partially eliminated inhibition by Pho, indicating that the inhibitory effect of Chlide, and to some extent Pho, was mediated through direct trapping of AFB1. Furthermore, spectrophotometric analysis showed that Pho treatment could increase GST activity in Hepa-1 cells. These observations indicate that the chlorophyll derivatives studied may attenuate AFB1-induced DNA damage in the Hepa-1 cell by direct trapping of AFB1. Pho provided additional protection not only by direct trapping, but also by increasing GST activity against hepatic AFB1 metabolites. |
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| Keywords: | Aflatoxin B1 Pheophorbide Glutathione S-transferase GST Chlorophyllide Chlorophyll derivatives |
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