Human milk lactoferrin binds two DNA molecules with different affinities. |
| |
Authors: | T G Kanyshkova D V Semenov V N Buneva G A Nevinsky |
| |
Institution: | Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences. |
| |
Abstract: | Evidence is presented that lactoferrin (LF), an Fe3+-binding glycoprotein, possesses two DNA-binding sites with different affinities for specific oligonucleotides (ODNs) (Kdl = 8 nM; Kd2 approximately 0.1 mM). The high affinity site became labeled after incubation with affinity probes for DNA-binding sites; like the antibacterial and polyanion-binding sites, this site was shown to be located in the N-terminal domain of LF. Interaction of heparin with the polyanion-binding site inhibits the binding of ODNs to both sites. These data suggest that the DNA-binding sites of LF coincide or overlap with the known polyanion and antimicrobial domains of the protein. |
| |
Keywords: | |
|
|