Quantitation of corneal fibril diameters from bony fish using low temperature preparative methods |
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| Institution: | 1. B-DAT, CICAEET, School of Information and Control, Nanjing University of Information Science and Technology, Nanjing 210044, China;2. Department of Informatics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom;3. Extreme Robotics Lab, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom;4. School of Information and Control Engineering, China University of Mining and Technology, Xuzhou 221116, China;1. Jiangsu Provincial Key Laboratory of Geographic Information Science and Technology, International Institute for Earth System Science, Nanjing University, Nanjing 210023, China;2. Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing 210023, China;3. Department of Geography, University of Toronto, Toronto, Ontario M5S3G3, Canada;4. Frontiers Science Center for Critical Earth Material Cycling, Nanjing University, Nanjing 210023, China;1. Department of Psychology, University of Utah, 380 S. 1530 E. BEHS 502, Salt Lake City, UT 84112, USA;2. Department of Psychology, University of New Mexico, MSC03 2220, Albuquerque, NM 87131, USA;3. The Mind Research Network, 1101 Yale Blvd. NE, Albuquerque, NM 87106, USA;4. Department of Neurosurgery, University of New Mexico, MSC10 5615, Albuquerque, NM 87131, USA;5. Department of Psychiatry, University of New Mexico, MSC 09 5030, Albuquerque, NM 87131, USA |
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| Abstract: | Low-temperature dehydration and embedding techniques have been used to preserve the transverse structure of corneal collagen fibrils from nine bony fish. The diameters measured all lie close to a value of 25.5 nm, in contrast to the smaller (and more diverse) diameters measured from “conventionally” prepared controls. The results are consistent with our earlier studies on the corneas from mammals, amphibians, birds, reptiles, and cartilaginous and bony fish which showed that the collagen fibrils from the bony fish were significantly smaller than those from animals of the other vertebrate classes. Thus, on the basis of the enhanced ability of the low-temperature preparative techniques to preserve collagen fibril structure the “in vivo” corneal collagen fibril diameters have been revised from 17 to 25.5 nm for bony fish (this work) and from 25 to 36 nm for all other classes of vertebrates (our previous work). |
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