Enhancement of pigment productivity of immobilized cultured Lavandula vera cells by limitation of nitrogen sources |
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| Affiliation: | 1. Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606, Japan;2. Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606, Japan;3. Research Institute for Polymers and Textiles, 1-1-4 Higashi, Tsukuba, Ibaraki 305, Japan;1. Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, United States;2. Dept. of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, United States;1. Movement Disorders Service and Section of Neurology, Institute for Neurosciences, St. Luke’s Medical Center, Quezon City, Philippines;2. Department of Neurosciences, College of Medicine, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines;3. Department of Clinical Epidemiology, College of Medicine, University of the Philippines Manila, Philippines;4. Section of Neurology, Institute for Neurosciences, St. Luke’s Medical Center, Quezon City, Philippines;5. Section of Endocrinology, Department of Medicine, St. Luke’s Medical Center, Quezon City, Philippines;1. Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia;2. Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia;3. Laboratory of Biomolecular Engineering, National School of Agronomy and Food Industries, National Polytechnics Institute of Lorraine ENSAIA-INPL, 54505, Vandoeuvre les Nancy, France |
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| Abstract: | Cultured cells of Lavandula vera entrapped with a photosensitive synthetic resin prepolymer (PVA-SbQ) produced blue pigments in the presence of l-cysteine as an inducer. The type of nitrogen sources in the culture medium greatly influenced the production of pigments. In the absence of an ammonium type nitrogen source, the induction of pigment synthesis by l-cysteine was observed in successive batches of the incubation without intermittent activation of the cells in the absence of l-cysteine. The pigment productivity of the entrapped cells was remarkably enhanced in the improved production medium containing potassium nitrate as the sole nitrogen source. |
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