Restoration of T-cell function in nude mice by grafting the epitheliomesenchymal thymic rudiment from 10-day-old euthymic embryos |
| |
Affiliation: | Institut d''Embryologie Cellulaire et Moléculaire du CNRS et du Collège de France, Nogent-sur-Marne, France |
| |
Abstract: | The capacity of the uncolonized thymic epithelium to restore immune function in nude mice was demonstrated by grafting the 3rd branchial arch area taken from euthymic 10-day BALB/c embryos into syngeneic newborn nude mice. Twenty-six percent of the operated animals became immunocompetent. T-cell function was tested with skin grafts and the presence of high levels of Thy-1 positive cells plus a variety of in vitro culture assays: Con A stimulation of T lymphocytes, cytotoxicity and alloreactivity in MLR of the recipient toward allogeneic spleen cells. All these tests showed a pattern of response similar to normal euthymic BALB/c mice. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |