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Production of cephalosporin C in a fluidized-bed bioreactor
Institution:1. Biochemical Process Laboratory, Genetic Engineering Center, The Korea Advanced Institute of Science and Technology, P.O. Box 131, Chongryang, Seoul, Korea;2. Department of Chemical Engineering, Seoul National University, Seoul 151, Korea;1. Natural Gas Engineering Department, School of Chemical and Petroleum Engineering, Shiraz University, Molla Sadra Street, Shiraz, 71348-51154, Iran;2. Center for Hydrate Research, Department of Chemical and Biological Engineering, Colorado School of Mines, Golden, CO, 80401, United States;1. Lodz University of Technology, Faculty of Process and Environmental Engineering, ul. Wólczańska 213, 90-942, ?ód?, Poland;2. Dortmund University of Technology, The Department of Biochemical and Chemical Engineering, Emil-Figge-Straße 70, D-44227, Dortmund, Germany
Abstract:Production of cephalosporin C was investigated in a fluidized-bed bioreactor using bioparticles of Cephalosporium acremonium. Bioparticles were developed by forming a biofilm of growing hyphae around celite particles which contained spores of the microorganism. Production of the antibiotic was significantly improved by using bioparticles over the free mycelial culture, possibly due to the enhanced mass transfer capacity of the bioreactor system and successive generation of highly productive morphological forms of the microorganism. The maximum attainable titer of cephalosporin C from the bioreactor system was almost double that from a jar fermentor operation with a free mycelial culture of the same strain. The biofilm of the bioparticles became unstable as the fermentation proceeded. Morphological differentiation of the microorganism caused a gradual loss of biofilm and an increase of free cells in the culture broth. Additional feeding of a limited amount of methionine to the fermentation broth was not as effective as expected for improving the bioparticle stability. However, repeated use of the bioparticles revealed a strong possibility to improved the overall reactor performance since it allowed an enhanced production of the antibiotic with fewer free cells.
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