Ethylene production from l-methionine by Cryptococcus albidus |
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| Affiliation: | 1. Laboratory of Plant Science, Migal – Galilee Technology Center, P.O. Box 831, Kiryat Shmona 12100, Israel;2. Tel-Hai College, Upper Galilee 11016, Israel;1. Enzymology and Fungal Biotechnology Lab, Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt;2. Applied Nucleic Acids Research Center, Chemistry Department, Faculty of Science, Zagazig University, Egypt;3. Biochemistry Department, Faculty of Agriculture, Zagazig University, Egypt;1. Crop Science Unit, AgroBioChem, Gembloux Agro-Bio Tech, University of Liege, Passage des Déportés 2, B-5030 Gembloux, Belgium;2. Plant Protection and Ecotoxicology Unit, Life Sciences Department, Walloon Agricultural Research Centre, Chemin de Liroux 2, B-5030 Gembloux, Belgium;1. Division of Viticulture and Pomology, Department of Crop Sciences, University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenz Straße 24, 3430 Tulln, Austria;2. Center for Analytical Chemistry, Department of Agrobiotechnology (IFA Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenz Straße 20, 3430 Tulln, Austria;1. AntiCancer Inc., San Diego, CA, USA;2. Department of Surgery, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, Seoul, South Korea;3. Department of Surgery, University of California, San Diego, CA, USA;4. Basic Research Laboratory, National Cancer Institute, Frederick, MD, USA |
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| Abstract: | Cryptococcus albidus IFO 0939 was selected from microorganisms producing ethylene from l-methionine in a culture medium. When methionine was excluded from the culture medium of C. albidus, there was little production of ethylene. Ethylene production in a methionine-containing culture medium occurred for a brief period at the end of the growth phase. 2-oxo-4-methylthiobutyric acid (KMBA), a deaminated product of methionine, accumulated in the culture filtrate. An ethylene-forming enzyme was partially purified from C. albidus by means of DEAE-Sepharose CL-6B ion exchange chromatography, and a cell-free ethylene-forming system was constructed. Using this system, the precursor of ethylene was found to be KMBA and essential factors were NAD(P)H, Fe3+, EDTA and oxygen. |
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