Isolation and characterization of carboxylesterase from vinyl acetate-assimilating bacterium isolated from soil |
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| Institution: | 1. Department of Cell Biology, Federal University of Paraná, Centro Politécnico s/No, Curitiba, PR 81.530-130, Brazil;2. Basic Bioscience Institute, University of Taubaté, Av. Tiradentes, 500-Centro, Taubaté, SP 12030-180, Brazil;3. Department of Marine Biology, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 373-CCS-Bloco A-Sala 89, Rio de Janeiro, RJ 21941-902, Brazil;1. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;2. Zhejiang Hisun Biomaterials Co., Ltd, Taizhou 318000, Zhejiang, China;3. College of Chemistry and Chemical Engineering, JiangXi Normal University, Nanchang 330022, China;1. Laboratory of Coastal Environmental Processes and Ecological Remediation, The Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;2. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;3. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;1. Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming 650500, China;2. Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, School of Life Sciences, Yunnan University, Kunming 650091, China;3. Key Laboratory of Yunnan Province for Biomass Energy and Environmental Biotechnology, Yunnan Normal University, Kunming 650500, China;1. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences (POI FEB RAS), Vladivostok, Russia;2. A.V. Zhirmunsky Institute of Marine Biology, National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences (NSCMB FEB RAS), Vladivostok, Russia |
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| Abstract: | A bacterium Pseudomonas species which is able to assimilate vinyl acetate and other esters as a sole source of carbon was isolated from soil. The bacterium contained three kinds of esterases (esterases I, II and III) which were separable on DEAE-cellulose column chromatography. Esterase II was purified and characterized. The enzyme is a monomer with the molecular weight of 27,000 and it hydrolyzed various esters most efficiently at pH 8.0. The activity of the enzyme was inhibited by diisopropylfluorophosphate. The purified enzyme was different from other esterases so far found in Pseudomonas sp. with respect to molecular structure and substrate specificity. |
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