Production of monoclonal antibody against protopectinase from Kluyveromyces wickerhamii |
| |
| Affiliation: | 1. Institute of Physics, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan;2. DHA Suffa University, Karachi 75500, Pakistan;3. Department of Physics, Rahim Yar Khan Campus, The Islamia University of Bahawalpur, Rahim Yar Khan 64200, Pakistan;4. Department of Physics, The Government Sadiq College Women University Bahawalpur-63100, Pakistan;5. Department of Physics, Government College University, Faisalabad,38000, Pakistan;6. Department of Physics, Zhejiang Normal University, Jinhua, Zhejiang, 321004, China;7. Department of Physics, Balochistan University of Information Technology, Engineering & Management Sciences, Quetta, 87300, Pakistan;8. Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia;9. Department of Physics, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, Saudi Arabia;10. Department of Physics, College of Arts and Science, Wadi Addawasir, Prince Sattam bin Abdulaziz University, Kingdom of Saudi Arabia;11. Department of Physics, Girl College for Art, Science, and Education, Ain-Shams University, Cairo, Egypt;12. Department of physics, College of Sciences, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia;1. Chemistry and Food Technologies Department, Polytechnic University of Madrid, Avenida Complutense S/N, 28040 Madrid, Spain;2. Gansu Key Laboratory of Viticulture and Enology, College of Food Science and Engineering, Gansu Agricultural University, 730070 Lanzhou, PR China;1. College of Material Science and Engineering, North China University of Science and Technology, Hebei, Tangshan 063210, China;2. College of Science, North China University of Science and Technology, Hebei, Tangshan 063210, China;3. School of Chemistry and Chemical Engineering, Tiangong University, Tianjin 300387, China;1. Department of Disease Control and Homeostasis, Kanazawa University Graduate School of Medical Sciences, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8641, Japan;2. Life Science Research Center, Kagawa University, Ikenobe 1750-1, Miki-cho, Kita-gun, Kagawa 761-0793, Japan;3. Division of Natural System, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan;4. Systems Life Sciences, Department of Medical Life Systems, Faculty of Medical and Life Sciences, Doshisha University, Kyotanabe, Kyoto 610-0394, Japan;5. Departments of Advanced Medicine, Kagawa University, Ikenobe 1750-1, Miki-cho, Kita-gun, Kagawa 761-0793, Japan;6. Department of Hospital Pharmacy, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8641, Japan;7. Department of Advanced Medical Technology, Kanazawa University Graduate School of Health Medicine, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8641, Japan;8. Division of Material Engineering, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan;9. Institute of Science and Engineering, Faculty of Natural System, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan;10. Department of Hospital Pharmacy, Kanazawa University Graduate School of Medical Sciences, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8641, Japan;11. Department of Medicinal Informatics, Kanazawa University Graduate School of Medical Sciences, 13-1 Takara-machi, Kanazawa, Ishikawa 920-8641, Japan;1. Mayo Clinic Vaccine Research Group, USA;2. Division of Computational Biology, Department of Quantitative Health Sciences, Mayo Clinic, Rochester, MN, USA |
| |
| Abstract: | Four monoclonal antibodies (MCA 3–6, 4-2, 9-2, and 10-2) against protopectinase (an endo-polygalacturonase) from Kluyveromyces wickerhamii were prepared. MCAs 3-6 and 4-2 reacted to protopectinases produced by K. fragilis and K. marxianus as well as to the protopectinase produced by K. wickerhamii. However, 9-2 and 10-2 were specific for the protopectinase from K. wickerhamii. These MCAs did not inhibit the protopectinase reaction or the polygalacturonase reaction of protopectinases produced by these species. We used these MCAs to find protopectinases in the culture filtrates of Kluyveromyces yeasts. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
