Production of peroxidase with horseradish hairy root cells in a two step culture system |
| |
| Affiliation: | 1. Center for Addiction Medicine, Massachusetts General Hospital, Boston, MA, USA;2. Department of Psychiatry, Harvard Medical School, Boston, MA, USA;1. Astrophysics Group, Imperial College London, Blackett Laboratory, Prince Consort Road, London, SW7 2AZ, UK;2. Universität Heidelberg, Zentrum für Astronomie, Institut für Theoretische Astrophysik, Albert-Ueberle-Strasse 2, Heidelberg,69120, Germany;3. University Observatory Munich, Scheinerstr. 1, Munich, D-81679 Germany;4. Max Planck Institute for Astronomy, Koenigstuhl 17, D-69117 Heidelberg, Germany;5. Department of Astronomy, University of Florida, Gainesville, FL, 32611, USA;6. Max-Planck-Institut für Extraterrestrische Physik, Giessenbachstrasse 1, Garching, D-85748, Germany;7. Centre for Astrophysics Research, Science and Technology Research Institute, University of Hertfordshire, Hatfield, AL10 9AB, UK |
| |
| Abstract: | Horseradish hairy root cells transformed by a soil bacterium Agrobacterium rhizogenes had peroxidase (POD) activity comparable to that of the original plant root tubers. To enhance POD production by the hairy root culture, various additives to the medium were tested including casein hydrolysates and plant extracts. Polypepton addition had a significant effect on the growth and POD production; at low concentrations (below 1 g/l) the growth was stimulated, while at high concentrations (3–10 g/l), POD activity in the cells was enhanced in spite of a low growth rate. Therefore, the hairy roots were at first cultured in the medium with 1 g/l Polypepton, and then 5 g/l Polypepton was added to enhance intracellular POD activity. POD activity in this two step culture system was 5.4 times higher than that in conventional culture in Polypepton-free medium. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
