The aging leydig cell: VII. Cytoplasmic estradiol receptors |
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Authors: | T Lin GCC Chen EP Murono J Osterman HR Nankin |
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Institution: | Medical Service, Wm. Jennings Bryan Dorn Veterans'' Hospital and Department of Medicine, University of South Carolina School of Medicine Columbia, South Carolina 29201 USA |
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Abstract: | Plasma estradiol and cytosolic estradiol receptor levels of testes were determined in a group of young (2–3 months) and old (24 months) Sprague-Dawley rats. Estradiol binding sites for the young rats averaged 5.6 ± 0.3 fmol/mg protein (), which was comparable to that of the old rats, 5.7 ± 0.3 fmol/mg protein (n=12). Using Scatchard analyses, the association constants at equilibrium of estradiol receptor binding of the old and young rats were the same, 6.1 × 1010M?1. Plasma estradiol levels were also similar in both groups-19.6 ± 2.8 pg/ ml (n=14) for the young and 19.2 ± 2.6 pg/ml (n=10) for the old rats. Our results suggest that impaired testosterone biosynthesis in old rats was not due to elevated plasma estradiol levels or to differences in testicular estradiol receptor content. |
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