Near-cognate peptidyl-tRNAs promote +1 programmed translational frameshifting in yeast |
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Authors: | Sundararajan A Michaud W A Qian Q Stahl G Farabaugh P J |
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Institution: | Department of Biological Sciences, University of Maryland, Baltimore County 21250, USA. |
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Abstract: | Translational frameshifting is a ubiquitous, if rare, form of alternative decoding in which ribosomes spontaneously shift reading frames during translation elongation. In studying +1 frameshifting in Ty retrotransposons of the yeast S. cerevisiae, we previously showed that unusual P site tRNAs induce frameshifting. The frameshift-inducing tRNAs we show here are near-cognates for the P site codon. Their abnormal decoding induces frameshifting in either of two ways: weak codon-anticodon pairing allows the tRNA to disengage from the mRNA and slip +1, or an unusual codon-anticodon structure interferes with cognate in-frame decoding allowing out-of-frame decoding in the A site. We draw parallels between this mechanism and a proposed mechanism of frameshift suppression by mutant tRNAs. |
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