Isolation and characterization of viruses from fetal calf serum |
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| Authors: | C. W. Molander A. J. Kniazeff C. W. Boone A. Paley D. T. Imagawa |
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| Affiliation: | (1) Hyland Division of Travenol Laboratories, University of California, San Diego;(2) National Cancer Institute, Bethesda, Maryland;(3) University of california, 90024 Los Angeles, California;(4) Present address: City of Hope Medical Center, 91010 Duarte, Calif |
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| Abstract: | Summary Ten lots of specially procured fetal calf serum collected under sterile conditions and not filtered and 16 lots of commercial fetal calf serum were tested for both human and bovine viral contamination. The presence of viruses was evaluated by observing for cytopathogenic effect (CPE), hemadsorption with guinea pig erythrocytes, and interference with cytopathogenic challenge viruses in both embryonic bovine trachea (EBTr) and human diploid lung (HDL) cells. Isolates were characterized by their cytopathogenicity, morphology, serology, and ability to propagate and produce cPE in a variety of bovine and nonbovine cells. One isolate was unequivocally identified as bovine herpes virus 1, and the other was presumptively identified as a bovine virus-diarrhea virus. This study was supported by The National Institutes of Health under Contract PH 43-66-539. |
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