Vegetative nuclei and differentiation of chromosomes inTrichophyton vanbreuseghemii

The stages of nuclear division were observed inTrichophyton vanbreuseghemii microcultures stained with Giemsa and by the Feulgen reaction. The course of karyokinesis is discussed. It takes the form of mitosis, modified by narrowing of the lumen of the hyphal cell and the movement of the cytoplasm. No spindle or centrioles were found. In vegetative hyphae (1.2–1.5 μm in diameter), the metaphase chromosomes were arranged lineally in a row following the direction of the long axis of the hyphal cell. The distribution of anaphase chromosomes occurs perpendicularly or obliquely to the cell walls. The chromosomes were spherical, with a diameter of about 0.3 μm. A haploid number of chromosomes (five) was found.