A specific inhibitor of Colletotrichum lindemuthianum protease from kidney bean (Phaseolus vulgaris) seeds |
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| Authors: | V V Mosolov M D Loginova E L Malova I I Benken |
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| Institution: | (1) A. N. Bach Institute of Biochemistry, Academy of Sciences of USSR, Leninski Prospect 33, W-71 Moscow, USSR;(2) N.I. Vavilov Institute of Plant Industry, Leningrad, USSR |
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| Abstract: | A specific protein—an inhibitor of Colletotrichum lindemuthianum protease—was isolated from kidney bean seeds in a homogeneous form. The purification procedure included gel filtration, isoelectric focusing and affinity chromatography on trypsin-Sepharose column. The latter was introduced to separate the fungal protease inhibitor from closely related trypsin and chymotrypsin inhibitors present in kidney bean seeds. |
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| Keywords: | Colletotrichum Host-pathogen relationships Phaseolus Protease inhibitors |
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