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Two osteoclastic markers expressed in multinucleate osteoclasts of goldfish scales |
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| Authors: | Azuma Kyoichi Kobayashi Masaki Nakamura Masahisa Suzuki Nobuo Yashima Sayaka Iwamuro Shawichi Ikegame Mika Yamamoto Toshio Hattori Atsuhiko |
| Affiliation: | a Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, Shinjuku-ku, Tokyo 169-8050, Japan b Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Ishikawa 927-0553, Japan c Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba 274-8510, Japan d Department of Oral Morphology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Okayama 700-8525, Japan e Department of Biology, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa, Chiba 272-0827, Japan |
| Abstract: | Complementary DNAs encoding two major osteoclastic markers, tartrate-resistant acid phosphatase (TRAP) and cathepsin K (Cath K) were cloned from the scales of a teleost, the goldfish. This is the first report of the full coding sequence of TRAP and Cath K molecules in fish. In the goldfish scale both TRAP and Cath K mRNAs were expressed in the multinucleate osteoclasts, which showed large numbers of mitochondria and lysosomes, and a well developed ruffled border. These characteristic features of osteoclasts in the scales are similar to those in mammals. Most teleosts use the scale as an internal calcium reservoir during the reproductive season. The expression of TRAP and Cath K mRNAs in the scale significantly increased in April, which is a reproductive season, compared with that in October, a non-reproductive season. Thus, both of these molecular markers should be useful for the study of osteoclasts in the teleost scale. |
| Keywords: | Tartrate-resistant acid phosphatase Cathepsin K Sequence Scale Bone Osteoclasts Morphology Goldfish |
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