首页 | 本学科首页   官方微博 | 高级检索  
     


pH modulation of transient state kinetics of enzymes. I. Simple theoretical models
Authors:J Ricard  M Crasnier
Affiliation:Centre de Biochimie et de Biologie Moléculaire du CNRS, BP 71, 13402 Marseille Cedex 9, France
Abstract:The effect of proton concentration on pre-steady-state kinetics has been investigated theoretically for enzyme reactions involving the breaking of one substrate into two products. Even for the simple double-intermediate mechanism the approach to the steady state may exhibit a rather complex kinetics, which is pH-dependent. This process may even exhibit damped oscillations. A change of pH may completely change this transient kinetics and even suppresses the oscillatory regime. A simple method is presented which allows estimation of the values of the rate and ionization constants. This procedure allows one to distinguish the simple double-intermediate mechanism from a more complex process where the 'fast' binding of the substrate induces a 'slow' conformation change of the enzyme.
Keywords:pH modulation  Transient state kinetics  Double-intermediate mechanism  Enzyme kinetics
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号