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无核白葡萄胚挽救育种技术研究
引用本文:李桂荣 王跃进 等. 无核白葡萄胚挽救育种技术研究[J]. 西北植物学报, 2001, 21(3): 432-436
作者姓名:李桂荣 王跃进 等
作者单位:1. 西北农林科技大学园艺学院
2. 新疆鄯善葡萄瓜果研究中心
基金项目:国家自然科学基金项目!( 39970 52 4 ),农业部 94 8项目!( 981 0 4 3)资助
摘    要:无核白葡萄在授粉后20-50d,将胚珠接种在含不同激素的Nitsch培养基上,培养80-90d后转入胚荫发培养基中,待胚萌发20d左右,再转入成苗培养基中使其发育成正常幼苗。结果表明,胚萌发适宜的培养基是附加0.5mg/L IAA,1.5mg/L BA和0.5mg/LGA3的Nitsh培养基,在所试10个接种时期中,无授粉后39d胚的发育率最高。

关 键 词:无核白葡萄 胚挽救 育种 无核品种
文章编号:1000-4025-(2001)03-0432-05
修稿时间:2000-11-20

The studies on embryo rescue techniques of'Thompson Seedless' grape
LI Gui rong,WANG Yue jin,TANG Dong mei,WANG Xi ping,LUO Qiang wei. The studies on embryo rescue techniques of'Thompson Seedless' grape[J]. Acta Botanica Boreali-Occidentalia Sinica, 2001, 21(3): 432-436
Authors:LI Gui rong  WANG Yue jin  TANG Dong mei  WANG Xi ping  LUO Qiang wei
Affiliation:LI Gui rong 1,WANG Yue jin 1*,TANG Dong mei 2,WANG Xi ping 1,LUO Qiang wei 2
Abstract:20~50 d after anthesis,ovules of 'Thompson Seedless' grape were cultured on Nitsch medium supplemented with different growth regulators and were transferred on germinating medium after 80~90 d cultured.20 d after the ovules were subcultured on 1/2 MS medium and plantlets were induced.The results indicated that the suitable medium was Nitsch+0.5 mg/L IAA+1.5 mg/L BA+0.5 mg/L GA3.The better period of inoculation was 39 days after anthesis.
Keywords:'Thompson Seedless' grape  rescue embryo
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