| 光学显微镜技术在活细胞单分子检测中的应用 |
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| 作者姓名： | Xu N Xu M Zhang YY |
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| 作者单位： | 北京大学第三医院血管医学研究所,分子心血管学教育部重点实验室,北京,100083 |
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| 基金项目： | This work was supported by the National Basic Research Priorities Programme of China (No. G2000056906), the National NaturalScience Key Program Foundation (No. 30490172) and the National Natural Science Foundation of China (No. 30200342). |
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| 摘 要： | 单分子检测是一门以高度的时间以及空间分辨率研究生物单分子的技术。近来,科学技术的探索发展使我们可以观察、检测甚至操纵单个分子并且研究它们的构象变化和动力学行为。这一发展使得以前被传统系综研究体系平均化所隐藏的新信息被揭示出来。单分子检测技术的发展已经揭开了生命科学研究的新篇章。在本文中,我们将介绍有关活细胞中单分子检测技术的发展以及活细胞内单分子检测的现状。
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| 关 键 词： | 单分子检测 活细胞 显微镜技术 分子检测技术 应用 光学 生命科学研究 空间分辨率 动力学行为 科学技术 构象变化 单个分子 研究体系 平均化 细胞内 |
Optical detection of single molecules in living cells |
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| Xu N,Xu M,Zhang YY.Optical detection of single molecules in living cells[J].Acta Physiologica Sinica,2005,57(3):271-277. |
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| Authors: | Xu Ning Xu Ming Zhang You-Yi |
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| Institution: | Institute of Vascular Medicine, Peking University Third Hospital and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing 100083, China. |
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| Abstract: | Single molecule detection is a technology of studying biomolecules with high spatial and temporal resolution. By exploitingrecent technical advances, we are able to observe, detect, even manipulate individual molecules and study their conformational changesand dynamic behaviors. New information can be obtained from the single molecule research, which is otherwise hidden or averaged out.In recent years, the development of single molecule detection techniques has opened up a new era of life science. In this review, weintroduce the advances of the techniques for detecting single molecules in cell biology and review the development of single moleculedetection in living cells. |
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| Keywords: | single molecules optical detection living cell |
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