Radioimmunoassay of prostaglandins E1, E2, and F2α in unextracted plasma, serum and myocardium |
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Authors: | Elizabeth J McCosh Donald L Meyer Jacqueline Dupont |
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Institution: | 1. Department of Food Science and Nutrition Colorado State University Fort Collins, Colorado 80523 USA |
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Abstract: | A radioimmunoassay procedure for the determination of PGE1, PGE2, and PGF2α is presented. The procedure involves the pre-precipitation of each prostaglandin specific antiserum with the precipitating antisera (ARGG), and the use of these antisera mixtures in assaying for PGE1, PGE2, and PGF2α. Applicability of the methods to unextracted plasma, serum and myocardial homogenate has been demonstrated through tests of specificity, recovery, reproducability and parallelism. A mathematical correction for cross-reactivity between PGE1 and PGE2, and their opposing antisera is given. To demonstrate the utility of the methodology in differentiation of experimental variables, prostaglandin concentrations in unincubated serum, incubated serum, and the rate of prostaglandin production in serum of dogs are given. |
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