Using eggshell membranes as a DNA source for population genetic research |
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Authors: | Krijn Baptist Trimbos Joyce Broekman Rosemarie Kentie Cees J M Musters and Geert R de Snoo |
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Institution: | (1) Institute of Environmental Sciences (CML), Leiden University, POB 9518, 2300 RA Leiden, The Netherlands;(2) Animal Ecology Group (CEES), University of Groningen, POB 14, 9750 AA Haren, The Netherlands |
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Abstract: | In the context of population genetic research, a faster and less invasive method of DNA sampling would allow large-scale assessments
of genetic diversity and genetic differentiation with the help of volunteer observers. The aim of this study was to investigate
the usefulness of eggshell membranes as a DNA source for population genetic research, by addressing eggshell membrane DNA
quality, degeneration and cross-contamination. To this end, a comparison was made with blood-derived DNA samples. We have
demonstrated 100% successful DNA extraction from post-hatched Black-tailed Godwit (Limosa limosa) eggshell membranes as well as from blood samples. Using 11 microsatellite loci, DNA amplification success was 99.1% for
eggshell membranes and 97.7% for blood samples. Genetic information within eggshell membrane DNA in comparison to blood DNA
was not affected (F
ST = −0.01735, P = 0.999) by degeneration or possible cross-contamination. Furthermore, neither degeneration nor cross-contamination was apparent
in total genotypic comparison of eggshell membrane DNA and blood sample DNA. Our research clearly illustrates that eggshell
membranes can be used for population genetic research. |
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