Callus Induction from Mesophyll and Hypocotyl Protoplasts of Mungbean (Vigna radiata (L))

Mesophyll and hypocotyl protoplasts were isolated from Vignaradiata using a combination of Cellulase Onozuka RIO (1%) andMacerozyme (0·2%). On a modified V-47 medium, 60% ofthe cultured protoplasts divided and developed into colonies.Protoclones differentiated roots on MS medium supplemented withdifferent auxins and cytokinins; however, shoot differentiationwas not obtained. Co-cultivation of protoplasts with wild andshooter mutants of Agrobacterium tumefaciens did not lead todifferentiation of plantlets. Lysopine and nopaline dehydrogenasewere not detected in any of the selected protoclones. Vigna radiata, Mungbean, Agrobacterium tumefaciens, co-cultivation, protoplasts