The signal transduction pathway of the nonintegrin receptor of 65 kDa is different from glycoprotein VI

The identity and signal pathways of a platelet nonintegrin receptor for type I collagen, 65 kDa, are not established. In this investigation, we have examined whether there is a difference in the signal transduction pathways between the 65-kDa protein and glycoprotein VI (GP VI). Results from this study show that these two proteins are different based on the following facts. First, the anti-65-kDa antibody does not precipitate GP VI and vice versa. Second, the Fc receptor (FcR) gamma chain which associates with GP VI after exposure to collagen does not associate with the 65-kDa protein. Third, tyrosine phosphorylation of the FcR gamma chain was obtained by Fab fragments of anti-GP VI but not by anti-65 kDa. These results suggest that the signal transduction pathway of the platelet receptors for the 65-kDa protein and GP VI are different.