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Distribution of DNA between sister cells during serial subcultivation of human fibroblasts |
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| Authors: | A. Macieira-Coelho A. Bengtsson M. Van der Ploeg |
| Affiliation: | (1) Department of Cellular Pathology, Institute of Cancerology and Immunogenetics, INSERM U 50 & Association Claude-Bernard, F-94800 Villejuif, France;(2) Division for Tumor Biology, The Wallenberg Institute, University of Uppsala, Uppsala, Sweden;(3) Department of Histochemistry and Cytochemistry, Sylvius Laboratories, Leiden University, NL-2333 AL Leiden, The Netherlands;(4) I.C.I.G., INSURM U 50, 14-16, av. Paul-Vailant-Couturier, F-94804 Villejuif Cédex, France |
| Abstract: | Summary The segregation of DNA at the time of cell division was analysed by measuring the DNA contents of cells in mitosis and the incorporation of tritiated thymidine (3H-TdR) on each half of anaphases and telophases. Results suggest that the spreads in the 2C and 4C DNA contents are due to quantitative differences which could originate during semi-conservative DNA synthesis, chromosome assembly and chromosome segregation. This continuous rearrangement of the genome could lead either to a degenerative process or to a differentiation program. |
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