Expression of chimeric genes in Caenorhabditis elegans |
| |
| Authors: | R A Jefferson M Klass N Wolf D Hirsh |
| |
| Affiliation: | 1. Department of Molecular, Cellular and Developmental Biology University of Colorado Boulder, CO 80309, U.S.A.;2. Department of Biology University of Houston Houston, TX 77004, U.S.A.;1. Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA;2. GROW School for Oncology and Developmental Biology, University of Maastricht, Maastricht, The Netherlands;3. Department of Pathology and Laboratory Medicine, Weill-Cornell Medicine, New York, NY, USA;4. Department of Pathology, Royal Women''s Hospital and VCS Foundation, Melbourne, VIC, Australia;5. Department of Pathology, Sibley Memorial Hospital, Johns Hopkins Hospital, Baltimore, MD, USA;6. Department of Pathology, Morristown Medical Center, Morristown, NJ, USA;7. Department of Pathology, Massachusetts General Hospital, Boston, MA, USA;8. Department of Diagnostic Pathology, Kumamoto University Hospital, Kumamoto, Japan;9. Department of Pathology, Belfast Health and Social Care Trust, Belfast, Northern Ireland, UK;10. The Jikei University School of Medicine, Tokyo, Japan;1. Retina Foundation of the Southwest, Dallas, Texas, USA;2. UT Southwestern Medical Center, Dallas, Texas, USA;3. Human Genetics Center, School of Public Health, University of Texas Health Science Center Houston (UTHealth), Houston, Texas, USA;4. Ruiz Department of Ophthalmology and Visual Science, McGovern Medical School, University of Texas Health Science Center Houston (UTHealth), Houston, Texas, USA;1. Department of Bioinformatics, Indian Institute of Information Technology Allahabad, Allahabad, 211015, UP, India;2. Department of Information Technology, Indian Institute of Information Technology Allahabad, Allahabad, 211015, UP, India;1. Department of Psychiatry and Biobehavioral Sciences, Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, Los Angeles, California;2. Department of Psychology, University of California, Los Angeles, Los Angeles, California;3. Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, California;4. Department of Neurobiology, University of California, Los Angeles, Los Angeles, California;5. Department of Psychiatry, University of Pittsburgh, Pittsburgh, Pennsylvania;6. Developmental Neurogenomics Unit, Human Genetics Branch, National Institute of Mental Health, Bethesda, Maryland |
| |
| Abstract: | We have shown the expression of transformed genes in the nematode Caenorhabditis elegans using a new gene fusion system. Vectors consisting of the flanking regions of a collagen gene (col-1) or a major sperm protein gene of C. elegans fused to the Escherichia coli uidA gene, encoding beta-glucuronidase, were microinjected into worms and found to be propagated as high-copy extrachromosomal tandem arrays. We have detected beta-glucuronidase activity in transformed lines, and have shown that the activity is dependent upon the correct reading frame of the construction and on the presence of the worm sequences. The enzyme activity was shown to be encoded by the chimeric beta-glucuronidase gene by co-segregation analysis and by inactivation with specific antisera. Expression is at a very low level, and seems to be constitutive. We have used histochemical techniques to visualize the enzyme activity in embryos. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
