Proposal of Desulfosarcina ovata subsp. sediminis subsp. nov., a novel toluene-degrading sulfate-reducing bacterium isolated from tidal flat sediment of Tokyo Bay |
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| Institution: | 1. Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido, Japan;2. Postdoctoral Research Fellow of the Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo 102-8471, Japan;3. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan;4. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan;5. National Institute of Technology, Kochi College, 200-1 Otsu, Monobe, Nankoku-city, Kochi 783-8508, Japan;1. Clinic for Birds, Reptiles, Amphibians and Fish, Justus Liebig University, Giessen, Germany;2. Institute of Hygiene and Infectious Diseases of Animals, Justus Liebig University, Giessen, Germany;3. Institute of Poultry Diseases, Department of Veterinary Medicine at the Freie Universität Berlin, Germany;1. School of Space and Environment, Beihang University, Beijing 100191, PR China;2. National Engineering Research Center of Coal Mine Water Hazard Controlling, Suzhou University, Suzhou 234000, PR China;3. School of Resources and Civil Engineering, Suzhou University, Suzhou 234000, PR China;4. Key Laboratory of Mine Water Resource Utilization of Anhui Higher Education Institute, Suzhou University, Suzhou 234000, PR China;5. Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Beihang University, Beijing 100191, PR China;1. Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo 186-8650, Japan;2. Yakult Honsha European Research Center for Microbiology ESV, Technologiepark 4, 9052 Zwijnaarde, Belgium;3. Department of Agricultural Sciences, University of Bologna, Viale Fanin 42, 40127 Bologna, Italy;4. Lactic acid Bacteria and Mucosal Immunity Team, Institut Pasteur de Lille, Rue Prof. Calmette, F-59019 Lille Cedex, France;5. Center for Infection and Immunity of Lille, F-59019 Lille, France;6. Université Lille Nord de France, F-59019 Lille, France;7. CNRS, UMR 8204, F-59019 Lille, France;8. Department of Animal Science and Technology, National Taiwan University, No. 50, Lane 155, Sec 3, Keelung Rd., Taipei 10673, Taiwan, ROC;1. School of Environmental Science and Engineering, Guangzhou University, Guangzhou, 510006, China;2. Key Laboratory for Water Quality Security and Protection in Pearl River Delta, Ministry of Education, Guangzhou, 510006, China;3. Guangdong Provincial Key Laboratory of Radionuclides Pollution Control and Resources, Guangzhou, 510006, China;4. School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China;1. Department of Aquatic Animals Disease, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Bursa, Turkey;2. Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain;3. Department of Aquatic Animal Disease, Faculty of Veterinary Medicine, Erciyes University, 38280 Kayseri, Turkey;4. Institut Mediterrani d’Estudis Avançats (IMEDEA, CSIC-UIB), Campus UIB, 07122 Palma de Mallorca, Spain |
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| Abstract: | Strain 28bB2TT is a sulfate-reducing bacterium isolated in a previous study, obtained from a p-xylene-degrading enrichment culture. Physiological, phylogenetic and genomic characterizations of strain 28bB2TT were performed to establish the taxonomic status of the strain. Cells of strain 28bB2TT were short oval-shaped (0.8–1.2 × 1.2–2.7 μm), motile, and Gram-negative. For growth, the optimum pH was pH 6.5–7.0 and the optimum temperature was 28–32 °C. Strain 28bB2TT oxidized toluene but could not utilize p-xylene. Sulfate and thiosulfate were used as electron acceptors. The G + C content of the genomic DNA was 53.8 mol%. The genome consisted of an approximately 8.3 Mb of chromosome and two extrachromosomal elements. On the basis of 16S rRNA gene analysis, strain 28bB2TT was revealed to belong to the genus Desulfosarcina, with high sequence identities to Desulfosarcina ovata oXyS1T (99.5%) and Desulfosarcina cetonica DSM 7267T (98.7%). Results of Average Nucleotide Identity (ANI) calculation and digital DNA–DNA hybridization (dDDH) analysis showed that the strain 28bB2TT should be classified as a subspecies under D. ovata. Based on physiological and phylogenetic data, strain 28bB2TT (=NBRC 106234 =DSM 23484) is proposed as the type strain of a novel species in genus Desulfosarcina, Desulfosarcina ovata subsp. sediminis subsp. nov. |
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| Keywords: | Sulfate-reducing bacteria Aromatic hydrocarbon degradation Mesophile |
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