Relationships of Bradyrhizobium strains nodulating three Algerian Genista species |
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| Institution: | 1. Laboratoire d’Ecologie Microbienne, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria;2. Biological Sciences, Binghamton University, Binghamton, NY 13902, USA;1. Laboratory of General and Agricultural Microbiology, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, Votanikos, 11855 Athens, Greece;2. Laboratory of Vegetable Production, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, Votanikos, 11855 Athens, Greece;1. CIRAD, UMR LSTM, F-34398 Cedex 5, Montpellier, France;2. IRD, UMR LSTM, F-34398 Cedex 5, Montpellier, France;3. LBVE, Badji Mokhtar University, BP 12, 23000 Annaba, Algeria;1. Laboratory of Legumes and Sustainable Agroecosystems, Centre of Biotechnology of Borj-Cédria, BP 901, Hammam-lif 2050, Tunisia;2. Agriculture and Agri-Food Canada, Vineland Station, Ontario L0R 2E0, Canada;3. Phytobacteriology Laboratory, Plant Protection Research Unit, CRRA Marrakesh, National Institute for Agronomical Research, Marrakesh 40000, Morocco;4. Research Support and Technology Transfer Unity, Centre of Biotechnology of Borj-Cédria, BP 901, Hammam-lif 2050, Tunisia;5. Laboratory of Microbial Biotechnologies, Agrosciences and Environment, Faculty of Science Semlalia, Cadi Ayyad University, Marrakesh 40000, Morocco;6. Agriculture and Agri-Food Canada, Ottawa, Ontario K1A 0C6, Canada;1. Laboratoire de Génétique, Biochimie et Biotechnologies Végétalesm, Faculté des Sciences de la Nature, Université Constantine 1, Route Ain El Bey 25000, Algeria;2. IRD – INRA LSTM, Tropical and Mediterranean Symbiosis Laboratory, Campus International de Baillarguet, TA A-82/J, 34398 Montpellier Cedex 5, France;1. Research Laboratory Biodiversity and Valorization of Arid Areas Bioresources (BVBAA) – Faculty of Sciences of Gabès, Erriadh, 6072, Tunisia;2. Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) – Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, Pozuelo de Alarcón, Madrid, 28223, Spain;3. Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, UPM, Madrid, 28040, Spain;4. Instituto de Ciencias Agrarias, CSIC, Madrid, 28006, Spain;1. State Key Lab of Agrobiotechnology, Ministry of Agriculture Key Lab of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China;2. Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, 11340 Mexico D. F., Mexico;3. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China |
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| Abstract: | The Mediterranean world is the cradle for the diversification of a large number of plant species, including legumes belonging to the Tribe Genisteae. Nodule bacteria from three species of Genista legumes indigenous to northwestern Africa (G. ferox, G. numidica, G. tricuspidata) were sampled across a 150 km region of Algeria in order to investigate symbiotic relationships. Partial 23S rRNA sequences from 107 isolates indicated that Bradyrhizobium was the predominant symbiont genus (96% of isolates), with the remainder belonging to Rhizobium or Mesorhizobium. A multilocus sequence analysis on 46 Bradyrhizobium strains using seven housekeeping (HK) genes showed that strains were differentiated into multiple clades with affinities to seven species: B. canariense (17 isolates), B. japonicum (2), B. ottawaense (2), B. cytisi/B. rifense (9), ‘B. valentinum’ (5), and B. algeriense (11). Extensive discordance between the HK gene phylogeny and a tree for four loci in the symbiosis island (SI) region implied that horizontal transfer of SI loci has been common. Cases of close symbiont relationship across pairs of legumes hosts were evident, with 33% of isolates having as their closest relative a strain sampled from a different Genista species. Nevertheless, tree permutation tests also showed that there was substantial host-related phylogenetic clustering. Thus, each of the three Genista hosts utilized a measurably different array of bacterial lineages. |
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| Keywords: | MLSA Phylogenetic characterization Root nodules Symbiosis |
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