In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves |
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Authors: | Alexander Schulz Christian Walther Helen Morrison Reinhard Bauer |
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Institution: | 1.Leibniz Institute for Age Research, Fritz Lipmann Institute;2.Friedrich Schiller University Jena;3.Institute of Molecular Cell Biology & Center for Sepsis Control and Care (CSCC) Jena University Hospital, Friedrich Schiller University Jena |
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Abstract: | Electrophysiological studies allow a rational classification of various neuromuscular diseases and are of help, together with neuropathological techniques, in the understanding of the underlying pathophysiology1. Here we describe a method to perform electrophysiological studies on mouse sciatic nerves in vivo.The animals are anesthetized with isoflurane in order to ensure analgesia for the tested mice and undisturbed working environment during the measurements that take about 30 min/animal. A constant body temperature of 37 °C is maintained by a heating plate and continuously measured by a rectal thermo probe2. Additionally, an electrocardiogram (ECG) is routinely recorded during the measurements in order to continuously monitor the physiological state of the investigated animals.Electrophysiological recordings are performed on the sciatic nerve, the largest nerve of the peripheral nervous system (PNS), supplying the mouse hind limb with both motoric and sensory fiber tracts. In our protocol, sciatic nerves remain in situ and therefore do not have to be extracted or exposed, allowing measurements without any adverse nerve irritations along with actual recordings. Using appropriate needle electrodes3 we perform both proximal and distal nerve stimulations, registering the transmitted potentials with sensing electrodes at gastrocnemius muscles. After data processing, reliable and highly consistent values for the nerve conduction velocity (NCV) and the compound motor action potential (CMAP), the key parameters for quantification of gross peripheral nerve functioning, can be achieved. |
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Keywords: | Neuroscience Issue 86 Demyelinating Diseases Neurodegenerative Diseases electrophysiology sciatic nerve mouse nerve conduction velocity neuromuscular diseases |
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