Long-term monoculture reduces the symbiotic rhizobial biodiversity of peanut |
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| Institution: | 1. Key Laboratory of Coastal Biology and Bioresource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China;2. Shandong Peanut Research Institute, Qingdao, 266100, China;3. Life Science College, Yantai University, Yantai, 264005, China;4. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;5. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China;6. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City D.F, 11340, Mexico;1. Laboratory of General and Agricultural Microbiology, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, Votanikos, 11855 Athens, Greece;2. Laboratory of Vegetable Production, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, Votanikos, 11855 Athens, Greece;1. Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil;2. Department of Biotechnology, Universidade Estadual de Londrina, C.P. 10011, 86057-970, Londrina, Paraná, Brazil;3. Centre for Rhizobium Studies (CRS), Murdoch University, 90 South St. Murdoch, WA, Australia;1. Universidade Estadual da Paraíba, R. Baraúnas, 351, Campina Grande, Paraíba, Brazil;2. Universidade Federal do Vale do São Francisco, Av. José de Sá Maniçoba, s/n, Petrolina, Pernambuco, Brazil;3. Universidade do Estado da Bahia, R. Edgard Chastinet, s/n, Juazeiro, Bahia, Brazil;4. Embrapa Algodão, R. Osvaldo Cruz, 1143, Campina Grande, Paraíba, Brazil;5. Embrapa Semiárido, BR 428, km 152, s/n, Petrolina, Pernambuco, Brazil;1. Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China;2. University of Chinese Academy of Sciences, Beijing, 100049, China;3. Institute of Research and Development, Duy Tan University, 03 Quang Trung, Da Nang, Vietnam |
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| Abstract: | Long-term monoculture (LTM) decreases the yield and quality of peanut, even resulting in changes in the microbial community. However, the effect of LTM on peanut rhizobial communities has still not been elucidated. In this study, we isolated and characterized peanut rhizobia from 6 sampling plots with different monoculture cropping durations. The community structure and diversity index for each sampling site were analyzed, and the correlations between a peanut rhizobium and soil characteristics were evaluated to clarify the effects on peanut rhizobial communities. The competitive abilities among representative strains were also analyzed. A total of 283 isolates were obtained from 6 sampling plots. Nineteen recA haplotypes were defined and were grouped into 8 genospecies of Bradyrhizobium, with B. liaoningense and B. ottawaense as the dominant groups in each sample. The diversity indexes of the rhizobial community decreased, and the dominant groups of B. liaoningense and B. ottawaense were enriched significantly with extended culture duration. Available potassium (AK), available phosphorus (AP), available nitrogen (AN), total nitrogen (TN) and organic carbon (OC) gradually increased with increasing monoculture duration. OC, TN, AP and AK were the main soil characteristics affecting the distribution of rhizobial genospecies in the samples. A competitive nodulation test indicated that B. liaoningense presented an excellent competitive ability, which was congruent with its high isolation frequency. This study revealed that soil characteristics and the competitive ability of rhizobia shape the symbiotic rhizobial community and provides information on community formation and the biogeographic properties of rhizobia. |
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| Keywords: | Peanut rhizobia Community Diversity Long-term monoculture Effect |
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