Arthrospiribacter ruber gen. nov., sp. nov., a novel bacterium isolated from Arthrospira cultures |
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| Affiliation: | 1. Department of Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, Abrahama 58, 80-307, Gdansk, Poland;2. Department of Pharmaceutical Microbiology, the Faculty of Pharmacy, Medical University of Gdansk, Al. Gen. Hallera 107, 80-416 Gdansk, Poland;3. Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland;1. IPREM UMR CNRS 5254, Université de Pau et des Pays de l’Adour, Avenue de l’Université, 64013 Pau Cedex, France;2. EPHE, UPVD-CNRS, USR3278 CRIOBE, PSL Research University, 66860 Perpignan, France;3. Sorbonne Université, CNRS, Laboratoire de Biodiversité et Biotechnologie Microbienne, LBBM, Observatoire Océanologique, 66650 Banyuls-sur-mer, France;4. Sorbonne Université, CNRS, Fédération de Recherche, Observatoire Océanologique, 66650 Banyuls-sur-mer, France;1. AixMarseille Univ., Université de Toulon, CNRS, IRD, MIO UM 110, 13288, Marseille, France;2. Université de Paris, Institut de Physique du Globe de Paris, CNRS UMR 7154, Paris, France;1. Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/no., 4169-007 Porto, Portugal;2. CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, Portugal;3. Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal;4. Department of Microbiology, Radboud University, Heyendaalseweg 135, Nijmegen, The Netherlands;5. Departamento de Ciências da Vida, Apartado 3046, Universidade de Coimbra, 3001-401 Coimbra, Portugal;6. Laboratório de Biologia Celular, Instituto de Ciências Biomédicas Abel Salazar, ICBAS, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal;7. Institute of Microbiology, Department of Microbial Interactions, Friedrich-Schiller University Jena, Philosophenweg 12, Jena, Germany;1. Department of Aquatic Animals Disease, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Bursa, Turkey;2. Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain;3. Department of Aquatic Animal Disease, Faculty of Veterinary Medicine, Erciyes University, 38280 Kayseri, Turkey;4. Institut Mediterrani d’Estudis Avançats (IMEDEA, CSIC-UIB), Campus UIB, 07122 Palma de Mallorca, Spain |
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| Abstract: | Polyphasic analysis of ten isolates of the red-pigmented bacteria isolated from ten Arthrospira cultures originating from different parts of the world is described. The 16S rRNA analysis showed <95 % identity with the known bacteria on public databases, therefore, additional analyses of fatty acids profiles, MALDI-TOF/MS, genome sequencing of the chosen isolate and following phylogenomic analyses were performed. Gram-stain-negative, strictly aerobic rods were positive for catalase, negative for oxidase, proteolytic and urease activity. Major fatty acids were 15 : 0 iso, 17:0 iso 3 OH and 17:1 iso w9c/16:0 10-methyl. The whole phylogenomic analyses revealed that the genomic sequence of newly isolated strain DPMB0001 was most closely related to members of Cyclobacteriaceae family and clearly indicated distinctiveness of newly isolated bacteria. The average nucleotide identity and in silico DNA–DNA hybridisation values were calculated between representative of the novel strains DPMB0001 and its phylogenetically closest species, Indibacter alkaliphilus CCUG57479 (LW1)T (ANI 69.2 % is DDH 17.2 %) and Mariniradius saccharolyticus AK6T (ANI 80.02 % isDDH 26.1 %), and were significantly below the established cut-off <94 % (ANI) and <70 % (isDDH) for species and genus delineation.The obtained results showed that the analysed isolates represent novel genus and species, for which names Arthrospiribacter gen nov. and Arthrospiribacter ruber sp. nov. (type strain DPMB0001 = LMG 31078 = PCM 3008) is proposed. |
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| Keywords: | ECE Low-income Workforce Classroom Quality |
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