Genotypic and symbiotic diversity of native rhizobia nodulating red pea (Lathyrus cicera L.) in Tunisia |
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| Affiliation: | 1. Laboratory of Legumes, Centre of Biotechnology of Borj-Cédria, BP 901, Hammam-lif 2050, Tunisia;2. Agriculture and Agri-Food Canada, Vineland Station, Ontario L0R 2E0, Canada;1. Universidade Federal do Vale do São Francisco (Univasf), Colegiado de Farmácia, Petrolina, PE, Brazil;2. Universidade Estadual da Paraíba (UEPB), Campina Grande, PB, Brazil;3. Universidade Federal Rural de Pernambuco (UFRPE), Departamento de Agronomia, Recife, PE, Brazil;4. Faculdade UniBras, Departamento de Agronomia, Juazeiro, BA, Brazil;5. Universidade de Pernambuco (UPE), Campus Petrolina, Petrolina, PE, Brazil;6. Universidade do Estado da Bahia (UNEB), Departamento de Tecnologia e Ciências Sociais, R. Edgard Chastinet, s/n, Juazeiro, BA, Brazil;7. Embrapa Semiárido, Petrolina, PE, Brazil;8. Fundação de Amparo à Pesquisa do Estado de Pernambuco (Facepe), Recife, PE, Brazil;9. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brasília, DF, Brazil;1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, 450000, P.R. China;2. Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Henan Province, 450000, P.R. China;3. Collaborative Innovation Center for Food Production and Safety of Henan Province, Zhengzhou 450002, Henan Province, P.R. China;4. LSTM, Univ Montpellier, CIRAD, INRAe, Institut Agro, IRD, Montpellier, France;5. Guyuan Branch of Ningxia Academy of Agricultural and Forestry Sciences, Guyuan 756000, Ningxia Province, P.R. China;6. State Key Laboratory of Agrobiotechnology, Beijing 100193, China; College of Biological Sciences and Rhizobium Research Center, China Agricultural University, Beijing 100193, P.R. China;7. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340 México, D. F., México;8. Department of Research and Development, Biotechnology, Uttaranchal University, Dehradun-248007, India;9. The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK;1. Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu 625014, China;2. Department of Environmental Sciences, University of Helsinki, Helsinki Fin-00014, Finland;3. Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China |
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| Abstract: | Nodulation and genetic diversity of native rhizobia nodulating Lathyrus cicera plants grown in 24 cultivated and marginal soils collected from northern and central Tunisia were studied. L. cicera plants were nodulated and showed the presence of native rhizobia in 21 soils. A total of 196 bacterial strains were selected and three different ribotypes were revealed after PCR-RFLP analysis. The sequence analysis of the rrs and two housekeeping genes (recA and thrC) from 36 representative isolates identified Rhizobium laguerreae as the dominant (53%) rhizobia nodulating L. cicera. To the best of our knowledge, this is the first time that this species has been reported among wild populations of the rhizobia-nodulating Lathyrus genus. Twenty-five percent of the isolates were identified as R. leguminosarum and isolates LS11.5, LS11.7 and LS8.8 clustered with Ensifer meliloti. Interestingly, five isolates (LS20.3, LS18.3, LS19.10, LS1.2 and LS21.20) were segregated from R. laguerreae and clustered as a separate clade. These isolates possibly belong to new species. According to nodC and nodA phylogeny, strains of R. laguerreae and R. leguminosarum harbored the symbiotic genes of symbiovar viciae and clustered in three different clades showing heterogeneity within the symbiovar. Strains of E. meliloti harbored symbiotic genes of Clade V and induced inefficient nodules. |
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| Keywords: | Symbiotic diversity Molecular diversity |
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