Characterization of Pisum sativum and Vicia faba microsymbionts in Morocco and definition of symbiovar viciae in Rhizobium acidisoli |
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| Institution: | 1. Centre de Biotechnologies Végétales et Microbiennes, Biodiversité et Environnement, Faculty of Sciences, Mohammed V University in Rabat, Morocco;2. Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), E-419, 18080 Granada, Spain;1. Key Laboratory of Resource Biology and Biotechnology in Western China, Taibai North Rd. 229, Xi''an City, Shaanxi Province, China;2. Department of Life Science, Northwest University, Taibai North Rd. 229, Xi''an City, Shaanxi Province, China;3. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340, Cd. México, Mexico;1. Centro de Ciencias Genómicas, UNAM, Cuernavaca, Morelos, Mexico;2. Laboratorio de Ecología Microbiana y Biotecnología, Departamento de Biología, Facultad de Ciencias, Universidad Nacional Agraria La Molina, Lima, Peru;3. Microbiomics Laboratory, ENES, UNAM, Morelia, Mexico;4. Instituto de Investigaciones Psicológicas, Universidad Veracruzana, Mexico;5. Centro de Investigaciones Gestálticas (CESIGUE), Mexico;6. Ecología Funcional, Instituto de Ecología, Jalapa, Veracruz, Mexico;1. National Centre for Microbial Resource, National Centre for Cell Science, Pune, Maharashtra 411007, India;2. Department of Biology, Queen''s University, Kingston, K7L 3N6, Canada;3. Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, 560012, India;1. Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, PO Box 2390, Marrakech, Morocco;2. Technische Universität Dresden, Institut für Genetik, Helmholtzstr. 10, D-01069 Dresden, Germany;3. Centre Régional de la Recherche Agronomique de Meknès, INRA, Morocco;4. Department of Biology, Faculty of Science and Techniques, BP. 509, 52000 Boutalamine, Errachidia, Morocco;1. Laboratory of Legumes, Centre of Biotechnology of Borj-Cédria, BP 901, Hammam-lif 2050, Tunisia;2. Agriculture and Agri-Food Canada, Vineland Station, Ontario L0R 2E0, Canada;1. Departamento de Microbiología y Genética and Instituto Hispanoluso de Investigaciones Agrarias (CIALE), Unidad Asociada Universidad de Salamanca- CSIC ‘Interacción Planta-Microorganismo’, Universidad de Salamanca, Edificio Departamental de Biología, Lab 209. Av. Doctores de la Reina S/N. 37007, Salamanca;2. Instituto de Investigación Biomédica de Salamanca (IBSAL), Complejo Asistencial Universitario de Salamanca, Universidad de Salamanca, CSIC, Salamanca, España;3. Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa;4. Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Salamanca, España |
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| Abstract: | In this work, we analyzed the diversity of seventy-six bacteria isolated from Pea and faba bean nodules in two regions of Morocco. The molecular diversity was realized using the analysis of the sequences of 16S rRNA and six housekeeping genes (recA, glnII, atpD, dnaK, rpoB and gyrB) and two symbiotic genes (nodA and nodC).The phylogeny of the 16S rRNA gene sequences revealed that all strains belong to the genus Rhizobium, being related to the type strains of R. leguminosarum, R. laguerreae, R. indigoferae, R. anhuiense and R. acidisoli. The housekeeping genes phylogenies showed that some strains formed a subclade distinct from the rhizobial species that usually nodulate Vicia faba and Pisum sativum which are closely related to R. acidisoli FH23 with sequence similarity of 98.3%.Analysis of the PGPR activities of the different isolates showed that the strains related to R. laguerreae were able to solubilize phosphates and to produce siderophores and auxin phytohormone. However, R. acidisoli strain F40D2 was unable to solubilize phosphates although they produce siderophores and IAA.The phylogenetic analysis of the nodA and nodC sequences showed that all isolated strains were closely related with the strains of symbiovar viciae. The nodulation tests confirmed the ability to nodulate V. faba and P. sativum but not Cicer arietinum or Phaseolus vulgaris. Hence, in Morocco P. sativum is nodulated by R. laguerreae; whereas V. faba is nodulated by R. laguerreae and the symbiovar viciae of R. acidisoli which has been not previously described in this species. |
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| Keywords: | Symbiosis Diversity Phylogeny |
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