Pseudomonas sivasensis sp. nov. isolated from farm fisheries in Turkey |
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| Affiliation: | 1. Department of Aquatic Animals Disease, Faculty of Veterinary Medicine, Bursa Uludag University, 16059 Bursa, Turkey;2. Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain;3. Department of Aquatic Animal Disease, Faculty of Veterinary Medicine, Erciyes University, 38280 Kayseri, Turkey;4. Institut Mediterrani d’Estudis Avançats (IMEDEA, CSIC-UIB), Campus UIB, 07122 Palma de Mallorca, Spain;1. CHU de Rennes, Service de Bactériologie-Hygiène hospitalière, Rennes, France;2. Université de Rennes 1, Unité Inserm U1230, Rennes, France;3. CH de Saint-Malo, Laboratoire de Biologie médicale, Saint-Malo, France;4. Univ Rennes, MRic TEM, CNRS, Inserm, BIOSIT - UMS 3480, US_S 018, Rennes, France;5. CHU Clermont-Ferrand, Laboratoire de Bactériologie & CNR de la Résistance aux Antibiotiques, Clermont-Ferrand, France;6. CNR de la Résistance aux Antibiotiques (laboratoire associé «Entérobactéries: résistance aux C3G et colistine»), Clermont-Ferrand, France;7. Université Clermont Auvergne, UMR INSERM 1071 USC INRA2018, Clermont-Ferrand, France;8. CNR de la Résistance aux Antibiotiques (laboratoire associé «Entérocoques»), Rennes, France;1. Department of Microbiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, SK, 94976, Nitra, Slovakia;2. Department of Bioenergy and Food Technology, Faculty of Biology and Agriculture, University of Rzeszow, Zelwerowicza St. 4, PL, 35601, Rzeszow, Poland;3. Institute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, K. Helmaņa iela 8, LV, 3004, Jelgava, Latvia;4. Department of Storing and Processing Plant Products, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, SK, 94976, Nitra, Slovakia;5. Department of Plant Genetics and Breeding, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia;1. Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy. University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain;2. Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain;3. Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología & Instituto CRETUS, Universidade de Santiago de Compostela, Santiago de Compostela, 15782 Spain;1. Laboratoire de Mycologie, Pathologies et Biomarqueurs, Département de Biologie, Université de Tunis ElManar, 2092 Tunis, Tunisia;2. Microbiologia, Departament de Biologia, Edifici Guillem Colom, Universitat de les Illes Balears, Campus UIB, 07122 Palma de Mallorca, Spain;3. INRA, UR 407, Unité de Recherche de Pathologie Végétale, Centre de Recherche Provence-Alpes-Côte d’Azur, Avignon, France;4. Laboratoire de Biotechnologie Appliquée à l’Agriculture, INRA Tunisia, Université de Carthage, 2094 Ariana, Tunisia;5. Institut Mediterrani d’Estudis Avançats (IMEDEA, CSIC-UIB), Campus UIB, 07122 Palma de Mallorca, Spain;1. Department of Biotechnology, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, Abrahama 58, 80-307, Gdansk, Poland;2. Department of Pharmaceutical Microbiology, the Faculty of Pharmacy, Medical University of Gdansk, Al. Gen. Hallera 107, 80-416 Gdansk, Poland;3. Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland;1. Department of Biology-Microbiology, Universitat de les Illes Balears, Crtra. Valldemossa km 7.5, 07122 Palma de Mallorca, Spain;2. Iranian Research Institute of Plant Protection, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran;3. Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Mazandaran, Sari, Iran;4. Institut Mediterrani d’Estudis Avançats (IMEDEA, CSIC-UIB), Crtra. Valldemossa km 7.5, 07122 Palma de Mallorca, Spain |
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| Abstract: | A study of 91 isolates from fish farms in Turkey showed that isolates P7T, P11, P24b, P29, P72, P73 and P158 belonged to the genus Pseudomonas according to 16S rRNA nucleotide sequence analysis. The analysis of the sequences of the RNA polymerase sigma factor gene (rpoD) located these strains in the Pseudomonas fluorescens lineage of species within the P. fluorescens subgroup, close to the cluster composed of the species Pseudomonas grimontii, Pseudomonas marginalis and Pseudomonas panacis. Based on similarities in the 16S rRNA and rpoD gene sequences of three previously isolated strains from other origins (CCUG 57209, CCUG 62357 and W5.2-93) linked them to the same cluster. A polyphasic taxonomic approach including phenotypic characterization, fatty acid composition, and multilocus sequence analysis, together with whole-cell MALDI-TOF data, corroborated this assumption. The genome G+C mol% contents were 59.48 and 59.71, respectively. The average nucleotide indices based on BLAST analysis and the genome-to-genome distance calculation for the P7T and CCUG 57209 strains with their closest relative, P. grimontii, were 88.16–88.29% and 38.10–38.20%, respectively. These data confirm that isolates P7T, P11, P24b, P29, P72, P73, P158, CCUG 57209, CCUG 62357 and W5.2-93 represent a new species for which the name Pseudomonas sivasensis is proposed, with P7T as a type strain (=CCUG 74260T= and CECT30107T). |
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| Keywords: | Aquaculture Water Rainbow trout |
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