Therapeutic effect of a TM4SF5-specific peptide vaccine against colon cancer in a mouse model |
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| Authors: | Sanghoon Kwon Young-Eun Kim Jeong-A Park Doo-Sik Kim Hyung-Joo Kwon Younghee Lee |
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| Institution: | 1.Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 200-702, Korea;2.Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju 361-763, Korea;3.Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea;4.Department of Microbiology, College of Medicine, Hallym University, Chuncheon 200-702, Korea |
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| Abstract: | Molecular-targeted therapy has gained attention because of its high efficacy and weak side effects. Previously, we confirmed that transmembrane 4 superfamily member 5 protein (TM4SF5) can serve as a molecular target to prevent or treat hepatocellular carcinoma (HCC). We recently extended the application of the peptide vaccine, composed of CpG-DNA, liposome complex, and TM4SF5 peptide, to prevent colon cancer in a mouse model. Here, we first implanted mice with mouse colon cancer cells and then checked therapeutic effects of the vaccine against tumor growth. Immunization with the peptide vaccine resulted in robust production of TM4SF5-specific antibodies, alleviated tumor growth, and reduced survival rate of the tumor-bearing mice. We also found that serum levels of VEGF were markedly reduced in the mice immunized with the peptide vaccine. Therefore, we suggest that the TM4SF5-specific peptide vaccine has a therapeutic effect against colon cancer in a mouse model. BMB Reports 2014; 47(4): 215-220] |
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| Keywords: | Animal model Colon cancer Peptide vaccine Therapeutics TM4SF5 |
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