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siRNA-mediated gene silencing of MexB from the MexA-MexB-OprM efflux pump in Pseudomonas aeruginosa |
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| Authors: | Feng-Yun Gong Ding-Yu Zhang Jiang-Guo Zhang Li-Li Wang Wei-Li Zhan Jun-Ying Qi Jian-Xin Song |
| Affiliation: | 1.Departments of Infectious Diseases, Huazhong University of Science and Technology, China;2.Anesthesiology, Wuhan Pu-ai Hospital, Tongji Medical College, Huazhong University of Science and Technology, China;3.Department of Infectious Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China;4.Department of Respiratory Medicine, Central Hospital of Qingdao, Qingdao 266042, Shandong, China |
| Abstract: | To gain insights into the effect of MexB gene under the short interfering RNA (siRNA), we synthesized 21 bp siRNA duplexes against the MexB gene. RT-PCR was performed to determine whether the siRNA inhibited the expression of MexB mRNA. Changes in antibiotic susceptibility in response to siRNA were measured by the E-test method. The efficacy of siRNAs was determined in a murine model of chronic P. aeruginosa lung infection. MexB-siRNAs inhibited both mRNA expression and the activity of P. aeruginosain vitro. In vivo, siRNA was effective in reducing the bacterial load in the model of chronic lung infection and the P. aeruginosa-induced pathological changes. MexB-siRNA treatment enhanced the production of inflammatory cytokines in the early infection stage (P ＜ 0.05). Our results suggest that targeting of MexB with siRNA appears to be a novel strategy for treating P. aeruginosa infections. [BMB Reports 2014; 47(4): 203-208] |
| Keywords: | Antibiotic sensitivity Efflux pump MexB Pseudomonas aeruginosa siRNA |