Analysis of Arabidopsis PsbQ A Gene Expression in Transgenic Tobacco Reveals Differential Role of Its Promoter and Transcribed Region in Organ-specific and Light-mediated Regulation |
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Authors: | Gaur Tripti Tyagi Akhilesh K |
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Institution: | Centre for Plant Molecular Biology, Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi-110021, India. |
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Abstract: | Arabidopsis PsbQ, encoding a 16 kDa protein of the oxygen-evolving complex, is regulated by light and is expressed preferentially in leaf tissues. To analyze the components required for light-regulated and organ-specific expression of PsbQA, several promoter constructs were generated and expressed in tobacco. The 2.2 kb promoter could confer organ-specific expression of the reporter gene, whereas regulatory elements for light-dependent induction could not be located within this promoter and the transcribed region extending up to a second exon, represented by a genomic fragment encompassing the gene. The genomic fragment representing the transcribed region, however, could confer light regulation even on a constitutive promoter, as observed by steady-state mRNA analysis in T0 and T1 tobacco plants. The results obtained have led to the conclusion that regulatory elements for organ-specificity mainly reside in the promoter region whereas the transcribed region of the gene has an important role in light regulation. |
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