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RNA sequencing suggests that non-coding RNAs play a role in the development of acquired haemophilia |
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| Authors: | Adrian Bogdan Tigu Ionut Hotea Rares Drula Alina-Andreea Zimta Noemi Dirzu Maria Santa Catalin Constantinescu Delia Dima Jon Thor Bergthorsson Victor Greiff Diana Gulei Daniel Coriu Margit Serban Johnny Mahlangu Ciprian Tomuleasa |
| Affiliation: | 1. Medfuture Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania Romanian Academy of Scientists, Bucharest, Romania Contribution: Data curation (equal);2. Department of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania Contribution: Data curation (equal);3. Medfuture Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania Romanian Academy of Scientists, Bucharest, Romania Contribution: Investigation (equal);4. Medfuture Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania Contribution: Investigation (equal);5. Medfuture Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania Contribution: Methodology (equal);6. Department of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania Contribution: Investigation (equal);7. Department of Hematology, Ion Chiricuta Clinical Cancer Center, Cluj Napoca, Romania Contribution: Investigation (equal);8. Biomedical Center, School of Health Sciences, University of Iceland, Reykjavík, Iceland Contribution: Investigation (equal);9. Department of Immunology, University of Oslo and Oslo University Hospital, Oslo, Norway Contribution: Investigation (equal);10. Department of Hematology, Carol Davila University of Medicine and Pharmacy, Cluj Napoca, Romania;11. Department of Pediatrics, Victor Babes University of Medicine and Pharmacy, Timisoara, Romania Contribution: Investigation (equal);12. Haemophilia Comprehensive Care Centre, Charlotte Maxeke Johannesburg Academic Hospital, University of the Witwatersrand and National Health Laboratory Service, Johannesburg, South Africa Contribution: Methodology (equal);13. Medfuture Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania |
| Abstract: | Acquired haemophilia (AH) is a rare disorder characterized by bleeding in patients with no personal or family history of coagulation/clotting-related diseases. This disease occurs when the immune system, by mistake, generates autoantibodies that target FVIII, causing bleeding. Small RNAs from plasma collected from AH patients (n = 2), mild classical haemophilia (n = 3), severe classical haemophilia (n = 3) and healthy donors (n = 2), for sequencing by Illumina, NextSeq500. Based on bioinformatic analysis, AH patients were compared to all experimental groups and a significant number of altered transcripts were identified with one transcript being modified compared to all groups at fold change level. The Venn diagram shows that haemoglobin subunit alpha 1 was highlighted to be the common upregulated transcript in AH compared to classical haemophilia and healthy patients. Non-coding RNAs might play a role in AH pathogenesis; however, due to the rarity of HA, the current study needs to be translated on a larger number of AH samples and classical haemophilia samples to generate more solid data that can confirm our findings. |
| Keywords: | acquired haemophilia coagulation epigenetics factor VIII RNA sequencing |