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Influence of cyclin D1 splicing variants expression on breast cancer chemoresistance via CDK4/CyclinD1-pRB-E2F1 pathway |
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| Authors: | Jing Wang Jiaxin Zhang Qinglong Ma Shasha Zhang Fengdie Ma Wei Su Taotao Zhang Xiaodong Xie Cuixia Di |
| Institution: | 1. School of Basic Medical Sciences, Lanzhou University, Lanzhou, China
Bio-Medical Research Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China Contribution: Conceptualization (lead), Data curation (lead), Formal analysis (lead), Writing - original draft (lead);2. School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, China Contribution: Data curation (supporting), ?Investigation (supporting), Methodology (supporting), Supervision (supporting);3. School of Basic Medical Sciences, Lanzhou University, Lanzhou, China Contribution: Data curation (supporting), ?Investigation (supporting), Methodology (supporting), Supervision (supporting);4. School of Basic Medical Sciences, Lanzhou University, Lanzhou, China Contribution: ?Investigation (supporting), Project administration (supporting), Supervision (supporting), Validation (supporting), Visualization (supporting), Writing - review & editing (supporting);5. Bio-Medical Research Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou, China Contribution: ?Investigation (supporting), Project administration (supporting), Supervision (supporting), Validation (supporting), Visualization (supporting), Writing - review & editing (supporting);6. School of Basic Medical Sciences, Lanzhou University, Lanzhou, China;7. Bio-Medical Research Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China |
| Abstract: | Cyclin D1 (CCND1), a mediator of cell cycle control, has a G870A polymorphism which results in the formation of two splicing variants: full-length CCND1 (CCND1a) and C-terminally truncated CCND1 species (CCND1b). However, the role of CCND1a and CCND1b variants in cancer chemoresistance remains unknown. Therefore, this study aimed to explore the molecular mechanism of alternative splicing of CCND1 in breast cancer (BC) chemoresistance. To address the contribution of G870A polymorphism to the production of CCND1 variants in BC chemoresistance, we sequenced the G870A polymorphism and analysed the expressions of CCND1a and CCND1b in MCF-7 and MCF-7/ADM cells. In comparison with MCF-7 cells, MCF-7/ADM cells with the A allele could enhance alternative splicing with the increase of SC-35, upregulate the ratio of CCND1b/a at both mRNA and protein levels, and activate the CDK4/CyclinD1-pRB-E2F1 pathway. Furthermore, CCND1b expression and the downstream signalling pathway were analysed through Western blotting and cell cycle in MCF-7/ADM cells with knockdown of CCND1b. Knockdown of CCND1b downregulated the ratio of CCND1b/a, demoted cell proliferation, decelerated cell cycle progression, inhibited the CDK4/CyclinD1-pRB-E2F1 pathway and thereby decreased the chemoresistance of MCF-7/ADM cells. Finally, CCND1 G870A polymorphism, the alternative splicing of CCDN1 was detected through Sequenom Mass ARRAY platform, Sanger sequencing, semi-quantitative RT-PCR, Western blotting and immunohistochemistry in clinical BC specimens. The increase of the ratio of CCND1b/a caused by G870A polymorphism was involved in BC chemoresistance. Thus, these findings revealed that CCND1b/a ratio caused by the polymorphism is involved in BC chemoresistance via CDK4/CyclinD1-pRB-E2F1 pathway. |
| Keywords: | alternative splicing breast cancer chemoresistance Cyclin D1 Cyclin D1b G870A polymorphism |