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The effects of patchouli alcohol and combination with cisplatin on proliferation,apoptosis and migration in B16F10 melanoma cells |
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| Authors: | Kai-Fu Chang Hung-Chih Lai Shan-Chih Lee Xiao-Fan Huang Ya-Chih Huang Tien-Erh Chou Chih-Yen Hsiao Nu-Man Tsai |
| Affiliation: | 1. Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan, R.O.C;2. Division of Hematology and Oncology, Department of Internal Medicine, Shin-Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, R.O.C Institute of Pharmacology, National Taiwan University, Taipei, Taiwan, R.O.C Contribution: Data curation (equal), Formal analysis (equal), Investigation (equal), Methodology (equal), Writing - original draft (equal);3. Department of Medical Imaging and Radiological Sciences, Chung Shan Medical University, Taichung, Taiwan, R.O.C;4. Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan, R.O.C Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, R.O.C Contribution: Methodology (equal), Software (equal);5. Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan, R.O.C Contribution: Data curation (equal), Formal analysis (equal);6. Division of Nephrology, Department of Internal Medicine, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chia-Yi, Taiwan, R.O.C |
| Abstract: | Melanoma is a highly metastatic cancer with a low incidence rate, but a high mortality rate. Patchouli alcohol (PA), a tricyclic sesquiterpene, is considered the main active component in Pogostemon cablin Benth, which improves wound healing and has anti-tumorigenic activity. However, the pharmacological action of PA on anti-melanoma remains unclear. Thus, the present study aimed to investigate the role of PA in the proliferation, cell cycle, apoptosis and migration of melanoma cells. These results indicated that PA selectively inhibited the proliferation of B16F10 cells in a dose- and time-dependent manner. It induced cell cycle arrest at the G0/G1 phase and typical morphological changes in apoptosis, such as chromatin condensation, DNA fragmentation and apoptotic bodies. In addition, PA reduced the migratory ability of B16F10 cells by upregulating E-cadherin and downregulating p-Smad2/3, vimentin, MMP-2 and MMP-9 expression. PA was also found to strongly suppress tumour growth in vivo. Furthermore, PA combined with cisplatin synergistically inhibited colony formation and migration of B16F10 cells and attenuated the development of resistance to treatment. Therefore, the results of this study indicate that PA may play a pivotal role in inducing apoptosis and reducing the migration of melanoma cells, and may thus be a potential candidate for melanoma treatment. |
| Keywords: | cell apoptosis drug combination melanoma migration patchouli alcohol |