Identification of effective leaf-rust resistance genes in wheat (Triticum aestivum) using STS markers |
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Authors: | L. G. Tyryshkin E. I. Gul’tyaeva N. V. Alpat’eva I. Kramer |
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Affiliation: | (1) Vavilov Institute of Plant Industry, St. Petersburg, 190000, Russia;(2) All-Russia Institute of Plant Protection, St.-Petersburg-Pushkin, 196608, Russia;(3) Institute of Epidemiology and Resistance Resources, Aschersleben, Germany |
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Abstract: | Molecular STS markers J13, Gb, and J09 were used for screening wheat (Triticum aestivum L.) accessions previously found to possess leaf-rust resistance genes according to test crosses or phytopathological tests. Specific amplification products were detected in all accessions assumed to possess the Lr9 gene, in nine of ten accessions with the conjectured Lr19 gene, and in 13 of 29 accessions with the conjectured Lr24 gene. Application of STS markers to identification of accessions possessing effective leaf-rust resistance genes is discussed. |
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