Polyphenol Characterization,Antioxidant and Skin Whitening Properties of Alnus cordata Stem Bark |
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Authors: | Antonella Smeriglio Valeria D'Angelo Marcella Denaro Domenico Trombetta Francesco Maria Raimondo Maria Paola German |
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Institution: | Antonella Smeriglio,Valeria D'Angelo,Marcella Denaro,Domenico Trombetta,Francesco Maria Raimondo,Maria Paola Germanò |
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Abstract: | In this study, we investigated the phenolic composition of the crude extract (MeOH 80 %) of Alnus cordata (Loisel .) Duby stem bark (ACE) and its antioxidant and skin whitening properties. RP‐LC‐DAD analysis showed a high content of hydroxycinnamic acids (47.64 %), flavanones (26.74 %) and diarylheptanoids (17.69 %). Furthermore, ACE exhibited a dose‐dependent antioxidant and free‐radical scavenging activity, expressed as half‐maximal inhibitory concentration (IC50): Oxygen radical absorbance capacity (ORAC, IC50 1.78 μg mL?1)>Trolox equivalent antioxidant capacity (TEAC, IC50 3.47 μg mL?1)>2,2‐Diphenyl‐1‐picrylhydrazyl (DPPH, IC50 5.83 μg mL?1)>β‐carotene bleaching (IC50 11.58 μg mL?1)>Ferric reducing antioxidant power (FRAP, IC50 17.28 μg mL?1). Moreover, ACE was able to inhibit in vitro tyrosinase activity (IC50 77.44 μg mL?1), l ‐DOPA auto‐oxidation (IC50 39.58 μg mL?1) and in an in vivo model it exhibited bleaching effects on the pigmentation of zebrafish embryos (72 h post fertilization) without affecting their development and survival. In conclusion, results show that A. cordata stem bark may be considered a potential source of agents for the treatment of skin disorders due to its bleaching properties and favorable safety profiles, associated to a good antioxidant power. |
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Keywords: | Alnus cordata Betulaceae mushroom tyrosinase zebrafish polyphenols RP-LC-DAD analysis antimelanogenic activity |
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