Influence of growth environment on the phosphoenolpyruvate: Glucose phosphotransferase activities of Escherichia coli and Klebsiella aerogenes: A comparative study |
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| Authors: | Neijssel O. M. Hardy G. P. M. A. Lansbergen J. C. Tempest D. W. O'Brien R. W. |
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| Affiliation: | (1) Laboratorium voor Microbiologie, Universiteit van Amsterdam, P.O. Box 20245, 1000 HE Amsterdam, The Netherlands;(2) Department of Biochemistry, University of Sydney, 2006 Sydney, N.S.W., Australia |
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| Abstract: | A consistent difference was found between glucose-limited cultures of Escherichia coli and Klebsiella aerogenes strains in the manner which their apparent cellular content of glucose: phosphoenolpyruvate phosphotransferase (glucose-PTS) varied with growth rate. With the former strains, activity increased as a function of growth rate; in the latter it decreased. However, under glucose-sufficient conditions (potassium-or ammonia-limitation) both species behaved similarly; the glucose-PTS activity was lower and bore no obvious relationship to the rate of glucose consumption expressed by the growing culture. These results are discussed in relation to the role of glucose as a regulator of glucose-PTS synthesis, and to the likely contribution which the glucose-PTS makes to the overall rate of glucose uptake, particularly by cells growing in glucose-sufficient environments.Abbreviation Glucose-PTS phosphoenolpyruvate phosphotransferaseFrom May to November 1978 on study leave in the University of Amsterdam |
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| Keywords: | Escherichia coli Klebsiella aerogenes Glucose uptake Glucose: Phosphoenolpyruvate phosphotransferase activities Chemostat culture Glucose-limitation Potassium-limitation Ammonia-limitation |
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