Resveratrol content and expression patterns of stilbene synthase genes in <Emphasis Type="Italic">Vitis amurensis</Emphasis> cells treated with 5-azacytidine |
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Authors: | K V Kiselev A P Tyunin A Y Manyakhin Y N Zhuravlev |
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Institution: | (1) Laboratory of Biotechnology, Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, 690022 Vladivostok, Russia;(2) Mountain-Taiga Station, Far East Branch of Russian Academy of Sciences, Posyolok Gornotaezhnoe, Primorsky Krai, 692533 Ussuriisky Region, Russia |
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Abstract: | DNA methylation is known to be involved in the regulation of plant development and defense mechanisms. However, there is a
general lack of data on the role of methylation in plant secondary metabolism. We have investigated the effect of a cytidine
analog, 5-azacytidine (azaC), which is known to block DNA methylation, on resveratrol biosynthesis and stilbene synthase (STS) gene expression in Vitis amurensis cultured cells. Resveratrol is a naturally occurring polyphenol that has been reported to exhibit a wide range of important
biological and pharmacological properties. We previously obtained a control cell line of V. amurensis (VV) as well as a rolB-transgenic cell line of V. amurensis (VB2) that has a higher level of resveratrol accumulation. In our experimental setup, the azaC-treated VV and VB2 calli produced
0.092% and 0.455% dry weight (DW) resveratrol, respectively. We found that treatment with 200 μM of azaC resulted in 1.9-
and 2.0-fold increases in resveratrol production in VV and VB2 calli, respectively. A quantitative real-time PCR assay for
STS gene expression in the azaC-treated VV and VB2 cells revealed that there were statistically increased expression levels of
VaSTS10 in VV calli and of VaSTS5, VaSTS6, and VaSTS10 in VB2 calli. These results demonstrate that azaC is able to increase resveratrol production in V. amurensis calli through a mechanism that involves the induction of STS gene expression. |
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