Inducible nitric oxide synthase mediates cytokine release: the time course in conscious and septic rats |
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Authors: | Lin Nien Tsung Yang Fwu Lin Lee Ru Ping Peng Tai Chu Chen Hsing I |
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Institution: | Department of Microbiology, Tzu Chi University, Hualien, Taiwan. |
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Abstract: | Nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), and interleukin 1-beta (IL-1beta) are postulated to play a key pathophysiologic role during sepsis. In this study, we examined the time course of inducible NO synthase (iNOS) mRNA expression and the plasma TNF-alpha and IL-1beta in lipopolysaccharide (LPS)-treated conscious rats. The hemodynamic pattern in septic shock is more similar to clinical conditions without anesthesia. The data showed that a significant increase in iNOS mRNA levels was found in the spleen, lung, liver, with slight elevation in the heart and kidney at 3 h after LPS administration. However, iNOS mRNA levels were not elevated significantly in all tissues examined at 24 h. In the plasma, TNF-alpha and IL-1beta culminated within 1 h, and reduced gradually to baseline levels in a relatively short period (within 9 h). The results suggest that local NO production by activation of iNOS mRNA expression and cytokine release may contribute to LPS-induced organ dysfunction at various time points. |
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Keywords: | Conscious rat Inducible nitric oxide synthase Endotoxin shock Cytokines |
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