温光敏核不育水稻N28S 无花粉败育的显微结构观察

采用石蜡切片和荧光显微技术观察了温光敏核不育水稻N28S 无花粉败育过程中的显微结构变化, 结果显示: N28S 的小孢子母细胞形成后细胞质变得稀薄, 一部分不能进行减数分裂, 一部分减数分裂阻滞在细线期或胞质分裂异常, 最终所有细胞液泡化解体消失。在此过程中, 还观察到小孢子母细胞在细线期胼胝质壁不产生或提早消失, 以及小孢子发育后期花药壁绒毡层的异常解体。认为N28S 的无花粉败育是由小孢子母细胞的细胞质异常引起的, 胼胝质壁和绒毡层的异常是结果而不是原因。

Microstructure Observations of Pollenless Abortion in Thermoand Photoperiod-sensitive Genic Male Sterile Line N28S in Rice ( Oryza sativa)

Using paraffin section method and fluorescence microscopy , anther microstructure changes of pollenless abortion were observed in thermo- and photoperiod-sensitive genic male sterile line N28S in rice. The results showed that the microsporocytes of N28S had a reduced cytoplasm. Some microsporocytes didn′t undergo meiosis, the other microsporocytes arrested meiotic division at the leptotene stage of meiosis or underwent abnormal cytokinesis . All abnormal microsporocytes and meiotic products became vacuolate and eventually degenerated, leaving empty anther locules . Moreover, the absence or premature degradation of callose wall around the microsporocytes and the abnormal degradation of tapetum in the microsporedevelopment stage were also been observed . It is suggested that the abnormal behaviour of callose and tapetum may be a consequence of a deviation in microsporocyte development, and the pollenless abortion of N28S is possibly attributed to microsporocytes with reduced cytoplasm .