提高狗蔷薇离体培养植株再生频率*

以狗蔷薇( Rosa canina Inermis) 为材料, 以MS 为基本培养基, 通过对不同植物生长调节剂的组合,
大幅度提高了狗蔷薇离体培养植株再生频率。结果表明, 210 mgPL 6-BA+ 01 3 mgPL 2, 4-D 的组合较为适宜,
其不定芽再生率为87%, 增殖率为310; 而CPPU 和2, 4-D 的适宜组合为115 mgPL+ 013 mgPL, 其不定芽再
生率高达93%, 增殖率为510。同时, 研究结果显示, 以MS+ 40 gPL 蔗糖+ 610 gPL 琼脂粉+ 31 5 mgPL
AgNO3 + 115 mgPL CPPU+ 01 1 mgPL 2, 4-D+ 0105 mgPL GA3 作增殖培养基效果最好, 不定芽诱导率为89%,
增殖率为51 5; 利于生根的培养基为1P4MS+ 20 gPL 蔗糖+ 315 gPL 琼脂+ 013%活性碳+ 011 mgPL IBA+ 011
mgPL NAA, 生根率为91%。

Improving of in vitro Plant Regeneration Frequency of Rosa canina ( Rosaceae) Inermis

An efficient regeneration system of Rosa canina Inermis was developed in this study. The results showed that
the regeneration frequency of adventitious buds was 87% and the multiplication ratio was 310 when supplemented with 210
mgPL 6-BA and 013 mgPL 2, 4-D onMurashige and Skoog (MS) medium, while the regeneration ratio was 93% and the
multiplication ratio was 510 when the MS medium contained 115 mgPL CPPU and 012 mgPL 2, 4-D. Moreover , the result
also indicated that CPPU was more effective in the induction of adventitious buds. The optimal medium for the multiplication
of shoots wasMS+ 40gPL sucrose+ 610 gPL agar+ 315 mgPL AgNO3 + 11 5mgPL CPPU+ 011 mgPL 2, 4-D+ 01 05mgP
L GA3 , in which the multiplication ratio was 515 and the adventitious bud induction frequency was 89%. The best med-i
um for rooting and growing was 1P4MS+ 20 gPL sucrose+ 31 5gPL agar+ 01 3% activated carbon+ 011 mgPL IBA+ 011mgP
LNAA. The rooting frequency was 91%.