苎麻细胞质雄性不育“三系”ISSR 特异片段克隆和序列分析

利用ISSR 分子标记技术对苎麻细胞质雄性不育“三系”mtDNA 进行多态性分析; 在选用的38 个ISSR引物中, 有6 个引物的扩增产物在不育系、保持系和恢复系之间存在差异。对这些特异性片段进行克隆和序列测定, 结果表明: 片段21-MS 全长956 bp , 包含一个525 bp 的完整编码区, 共编码174 个氨基酸。片段31-M􊄯R 全长778 bp , 包含一个404 bp 的不完整编码区, 共编码134 个氨基酸; 其核苷酸和氨基酸序列与已报道的多种植物中的番茄红素β-环化酶基因分别存在71%～76%和73%～77%的同源性。

Cloning and Sequencing for ISSR Specific Fragments in Cytoplasmic Male Sterile,Maintainer and Restorer Lines of Ramie ( Boehmeria nivea)

The polymorphic analysis of mtDNA from ramie cytoplasmic male sterile (MS) , maintainer (M) and restorer (R) lines ( three lines) were performed by using ISSR technique . Thirty-eight ISSR primers were used for testing mtDNApolymorphism and different amplified bands were observed in six primers in ramie“three lines”, these specific bands were cloned and sequenced . Among those bands, the fragment 21-MS (GenBank accession number : EU122345) had 956 bp in total length and a 525 bp intact coding region that encoded a polypeptide consisting of 174 amino acids . Fragment 31-M􊄯R(GenBank accession number : EU122344) had 778 bp in total length and a 404 bp incompletely coding region that encoded a polypeptide consisting of 134 amino acids; this fragment had 71%- 76% and 73%- 77% of homology in nucleotide and amino acid sequences with lycopene beta-cyclase allozyme gene in many other plants .